Who Uses NAD+
Energy & Focus
Fast-acting mental clarity and physical energy — one of the first things people feel.
Longevity
The foundational anti-aging protocol — restores levels that drop off after 40.
High Performers
Athletes and executives use it for endurance, recovery, and cognitive sharpness.
1
Reconstitution
Vial Size & Water Guide
| Vial Size | Bacteriostatic Water | Concentration | 100mg Dose → Draw To |
|---|---|---|---|
| 500 mg | Add 2 mL | 2.5 mg per unit | 40 units on the syringe |
| 1000 mg | Add 4 mL | 2.5 mg per unit | 40 units on the syringe |
Quick reference: 50mg = 20 units | 100mg = 40 units | 250mg = 100 units (both vial sizes)
Tilt the vial and inject bacteriostatic water slowly down the glass wall. Swirl gently until dissolved — do not shake. Refrigerate immediately after mixing.
Watch: How to Reconstitute a Peptide Vial
Note: water amounts vary by compound — always follow the reconstitution amounts listed in the table above for NAD+.
2
How to Inject
Method
SubQ or IM
SubQ: belly fat | IM: outer thigh
Needle Angle
45° SubQ / 90° IM
Pinch skin for SubQ; relax muscle for IM
Preferred Sites
Belly fat or outer thigh
Rotate sites daily
Timing — Important
Morning only
NAD+ is energizing — injecting at night may disrupt sleep
3
Your Protocol
Slow & Steady
50 mg
per day
Best For
First-time injectable NAD+ users, gentle cellular energy boost
Draw Amount
50mg → draw to 20 units
(both vial sizes)
(both vial sizes)
Protocol
50mg/day — maintain throughout
Most Popular
Suggested
100 mg
per day
Best For
Most users — strong cellular effects, energy, mental clarity, and metabolic support
Draw Amount
100mg → draw to 40 units
(both vial sizes)
(both vial sizes)
Titration
Wk 1: 50mg/day → Wk 2+: 100mg/day
Aggressive but Safe
250 mg
per day
✓ Used by experienced biohackers — well-tolerated in community
Best For
Experienced users, deep cellular repair, longevity optimization
Draw Amount
250mg → draw to 100 units
(both vial sizes)
(both vial sizes)
Titration
Wk 1: 50mg → Wk 2: 100mg → Wk 3+: 250mg/day
Titration Comparison — All 3 Protocols Side by Side (Daily Dose)
| Week | Slow & Steady | Suggested | Aggressive |
|---|---|---|---|
| Week 1 | 50 mg | 50 mg | 50 mg |
| Week 2 | 50 mg | 100 mg | 100 mg |
| Week 3+ | 50 mg | 100 mg | 250 mg |
What to Expect
- Days 1–3: Increased energy, mental clarity, and improved focus. One of the fastest-acting effects of injectable NAD+.
- Week 1–2: Improved metabolic rate, better sleep quality, and a noticeable uptick in physical endurance.
- Long-Term (weeks–months): Cellular repair accumulates. Anti-aging effects, improved DNA repair efficiency, and sustained energy improvements.
Do
- Inject in the morning — it's stimulating and may affect sleep if dosed late
- Stay hydrated — NAD+ supports increased cellular metabolism
- Pair with resveratrol or NMN for potential synergistic longevity effects
- Common: 5 days on, 2 days off cycle
Don't
- Inject at night — too energizing, will likely disrupt sleep
- Expect effects to be dramatic at first — they build over time
- Shake the vial — swirl gently only
- Store at room temperature after reconstitution
↗
Supplies You'll Need
Insulin Syringes (for injection)
Use one fresh syringe per injection. 29–31 gauge ensures minimal discomfort for SubQ use.
Reconstitution Syringe
For mixing only — do NOT use for injection.
Alcohol Swabs
Wipe the vial top and injection site before every use.